Lambin attended Cypress Falls High School. As a third baseman, Lambin was named to the Baseball Factory Pre-Season First Team. Playing baseball for Grayson County College, Lambin was named to the JUCO World Series All-Tournament Team in 1999 and 2000. He spent one season at the University of Texas at Austin, hitting for a .214 batting average in 28 at-bats.

Lambin transferred to the University of Louisiana at Lafayette, where he played as a senior for the Louisiana–Lafayette Ragin' Cajuns baseball team. He was selected by the New York Mets in the 34th round (1017th overall selection) of the 2002 Major League Baseball Draft.

Lambin played in the Mets' minor league organization from 2002 through 2006. In 2005, he had a .309 batting average and 24 home runs between the Binghamton Mets of the Class AA Eastern League and the Norfolk Tides of the Class AAA International League. In 2006, his batting average regressed to .222. He was demoted from Norfolk to Binghamton, and released during spring training in 2007.

In 2007, Lambin played for the Class AA Carolina Mudcats. In 116 games, he hit .283 with 15 home runs and 53 RBI. The next year, Lambin was promoted to the Class AAA Albuquerque Isotopes, where he hit .300 with 14 home runs and 54 RBI.

In 2009, Lambin played for the Chiba Lotte Marines in Japan's Pacific League under manager Bobby Valentine. Sportswriter Mike Waters wrote that the season in Japan "combined the worst season of baseball in Lambin’s career with perhaps the best year in his life. He hit .192 with 4 home runs and 12 runs batted in (RBI) in 58 games.

Lambin blogged about his experiences in Japan and later commented, "Even the writing doesn’t capture how amazing it was. I'd write about it and I'd tell my parents and family and friends and even my parents didn't really grasp it until they came over and saw it for themselves. It was a magic carpet ride the whole time.

Lambin returned to the United States in 2010, where he played for the Washington Nationals organization. He was named the "Top Star" for the International League in the 2010 Triple-A All-Star Game. He hit .252 with 15 home runs and 58 RBI that year. The next year, playing for the Rochester Red Wings (the Class AAA affiliate of the Minnesota Twins), he hit .274 with 6 home runs and 46 RBI.

On February 15, 2012, Lambin signed a minor league contract with the Marlins. He appeared in 89 games for the Class AAA New Orleans Zephyrs and hit for a .253 batting average with 8 home runs and 32 RBI. He also pitched one scoreless inning that season. After being released by the Marlins, he signed with the independent Sugar Land Skeeters. On May 30, 2013, he signed a minor league contract with the Kansas City Royals. As of July 2013, Lambin played for the Class AAA Omaha Storm Chasers and was the oldest active minor leaguer to never have played in MLB.